The collection of 640 samples of breeding varieties and landraces from more than 30 countries around the world, most of the European countries - 58.9% is created. The accessions are documented in the National Catalogue of Plant Genetic Resources of Ukraine; information about them is included in the European Catalogue of Plant Genetic Resources EURISCO. There is carried out study of potato accessions on productivity, resistance to biotic and abiotic environmental factors. We study the heat- and drought-resistance of the varieties. In the natural and provocative infectious backgrounds, potato varieties are being assessed for resistance to viruses. As a result of perennial researches, 5 trait collections were formed and registered. Allocated sources and donors of agronomic traits are allocated each year and transmitted to users for use in breeding, scientific and educational programs.