0ne of the most important issues of breeding is development of new varieties in which to assemble more valuable characters, high productivity, increased resistance to unfavorable environmental factors and precocity with large and qualitative fruits. However, to accelerate the process of creating such varieties, it is necessary to apply new selection methods and technologies.

We mentioned that a real perspective in this aspect was presented by the modern biotechnology methods and techniques, based on the principles of molecular genetics, genetic and cellular engineering, cell cultures and vegetal tissues, accelerated micro-propagation, induced variability and somaclonal variability, remote hybrids.

Remote hybridization methods with the mobilization of the spontaneous genofond and the application of the in vitro culture allowed us to settle many issues in the amelioration process. Through classical hybrids, ef icient capacities have been transferred to the activity of plant regeneration by crossing spontaneous species with the crop species through the embryo calus culture.

The hybridization of different genetically-stable species varied according to the degree of kinship between them, with the transmission of genetic information that results in some valuable economic carcasses from spontaneous species to culture species. Thus, the emergence of biotechnologies, especially in vitro culture, produced a real revolution in the process of improving vegetable crops and other agricultural species.