Among the different methods of improvement of the valuable characteristics of tomato (Solanum lycopersicum) cultivars, the genetic engineering, in particular, the gene editing technology or Agrobacterium-mediated transformation, are of continued interest up to date. To enhance the efficiency of the Agrobacterium-mediated transformation of tomato, different approaches could be applied, one of them is the use of the most relevant combination of phytohormones in the selective medium facilitating the highest plant regeneration frequency in vitro. In this study the influence of such synthetic plant growth regulators as Ivin, Metiur and Kametur on the frequency of Agrobacterium-mediated tomato transformation was studied. The 10-day-old seedlings of tomato cvs. ‘Money Maker’ and ‘Lahidny’ were transformed with the use of A. tumefaciens EHA105 carrying pBin35LF plasmid. Ivin, Metiur and Kametur at concentrations 0,1, 1 and 10 μМ were added into the selective medium MST (Buziashvili et al., 2020) supplemented with 100 mg/l kanamycin, 1 mg/l zeatin and 1 mg/l indole-acetic acid (IAA), or with 100 mg/l kanamycin, 2 mg/l BAP, 0.5 mg/l IAA. After 3 months of selection, it was shown that in the presence of 0.1 μM Metiur, 1 mg/l zeatin, 1 mg/l IAA the transformation frequency was at 8.89% for tomato cv. ‘Lahidny’ and 7.04% for tomato cv. ‘Money Maker’, and these values were higher than in the absence of growth stimulators – 3.87% for cv. ‘Lahidny’ and 2.5% for cv. ‘Money Maker’, respectively. Also, plant regeneration on explants of tomato cv. ‘Money Maker’ in the presence of 2 mg/l BAP, 0.5 mg/l IAA, 0.1 μM Metiur was observed, and the transformation frequency was at 2.02% on this selective medium, while on the medium containing only 2 mg/l BAP, 0.5 mg/l IAA the plant regeneration on explants of tomato cvs. ‘Lahidny’ and ‘Money Maker’ was not observed.