Grapevines (Vitis spp.) are affected by many viral diseases causing serious pathological problems. Among the most widespread leafroll viruses is GLRaV-3 meanwhile, grapevine fanleaf virus (GFLV) is a destructive viral disease that reduce longevity of vineyards. Considering the impact and the spread of these diseases, we have analysed for viral presence several grapevine varieties in grapevine collection at ATTC Vlorë, in order to estimate whether it is feasible sampling for vegetative propagation, due to mandatory rules for usage of certified plant material for propagation purposes, as an effective way to prevent spread of pathogens. The presence of two common viruses were tested using virus specific primers; LC1/LC2 primer pair designed in the hHSP70 gene for detecting Grapevine Leafroll-associated Virus-3 (GLRaV3) and Grapevine Fanleaf Virus (GFLV) was tested with C3390/H2999 primer pair, in six varieties: ‘Merlot’, ‘Kallmet’, ‘Shesh i zi’, ‘Shesh i bardhë’, ‘Debinë’, ‘Pulëz’, provided through a randomised sampling procedure.  One Step Reverse Transcription Polymerase Chain Reaction assay was used to detect presence of two viral diseases. The results showed a high prevalence of GLRaV3 virus (100%) in all of samples analyzed, resulting as the most outspread infection among analyzed samples. Analysis for the presence of GFLV virus showed low infection rate, being present in only one sample. This method is efficient, fast and reproducible for detecting grapevine viruses through one step RT- PCR. These results point out that due to presence of viral infections detected, it’s not recommended sampling of infected plant material for vegetative reproduction.